Abstract
Vertically interrogated porous silicon (PSi) interferometric biosensors have shown high potential for sensing bio-molecules as they combine high detection sensitivity with simplicity of fabrication, functionalization, optical coupling, and interfacing with microfluidic systems. However, most interferometric sensor designs require either broadband or wavelength-tunable light sources as well as wide-angle detection schemes, increasing their complexity and cost for point-of-care biosensing applications. The limit of detection of such sensors is also constrained by the small size and low refractive index of biological molecules, making it hard to detect very low concentrations of pathogens. In this work, we use a large-scale computational “inverse design” technique to demonstrate a single-frequency, fixed-angle PSi-based biosensor, which exploits a recently developed high-contrast reporter cleavage detection (HCCD) technique. The HCCD sensors detect high-index reporter cleavage events instead of low-index target analyte capture events as typical for traditional label-free optical biosensors. We use the inverse design approach to discover an optimal configuration of a PSi biosensor that makes use of the extended achievable range of cleavage-induced PSi effective index variations and can be interrogated at a single frequency and at a fixed angle. The optimized design in the form of a one-dimensional PSi grating exhibits the change in the reflectance up to 55 % at the interrogation angle of ${12}^{\circ}$ and wavelength of 600 nm, which is caused by cleavage of Au nanoparticle reporters initially occupying 2% of the sensor surface area. The maximum possible change in reflectance is predicted to be 222 % (for a two-dimensional freeform design not amenable to fabrication). This demonstration may pave the way for developing new or redesigned conventional interferometric and colorimetric point-of-care biosensor systems in combination with the cleavage-based detection schemes.
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
1. Introduction
Porous silicon (PSi)-based optical biosensors have demonstrated great potential for applications in commercialized real-time sensing of biomolecules [1–11]. PSi offers a variety of advantages in optical biosensing, including extremely large surface area, fabrication simplicity, and well-developed surface chemistry for device functionalization with receptors to capture biological molecules [12]. Many practical PSi biosensor platforms have been developed, including single-layer interferometers [13,14], Bragg mirrors [15], microcavities [6], Rugate filters [5], diffraction gratings [9,16,17], ring resonators [4], and colorimetric sensors [18]. The PSi-based optical biosensor design approaches have recently evolved towards engineering relatively high-quality factor photonic devices capable of detection of very small changes of refractive indices caused by the capture of target bio-molecules [4,12]. However, it opens a new challenge, where a small noise can be also amplified in a high-quality factor design. These includes the spectral noise due to the limited spectral resolution of both the laser and the optical detection system, thermal variations, and the indiscriminate molecular binding events [19,20]. While the effect of the thermal noise can be reduced by referencing or self-referencing the biosensor [21,22], increasing spectral resolution of the system also increases its cost and complexity, and the indiscriminate binding events are hard to avoid when working with biological molecules.
On the other hand, low-quality factor optical biosensor designs can still achieve low detection limits provided they can be engineered to achieve high sensitivity to the refractive index changes in the environment [19,23]. The diffraction grating is a particularly interesting low-quality-factor optical biosensor system, because it only requires a single frequency light source and a photodetector, eliminating the need for complicated broadband high-spectral-resolution signal processing, which could have a great advantage in developing a simple real-time point-of-care sensing systems. Two types of grating-based biosensors have been previously proposed and investigated. A grating-coupler waveguide approach uses the angular-dependent response of the grating coupler, where light incident at the critically-matched angle creates a strong resonance in the reflectance signal [9]. On the other hand, a diffraction-grating approach is based on measuring the changes in the intensity of light reflected at a fixed wavelength and into a fixed angle as a result of light diffraction on a grating structure [24,25]. The latter approach only requires a single-frequency or a narrowband light source illuminating the grating at a fixed angle, and a single photo-detector to measure the reflected signal, making it a great candidate for the simplest design of a point-of-care biosensor.
However, creating clear distinct diffraction intensities for the biosensor response corresponding to the positive and negative test results has been very challenging due to a low refractive index of biological molecules and the difficulty of designing multi-state diffractive optical devices. Recently, we have proposed and experimentally demonstrated the High-Contrast Cleavage Detection (HCCD) technique on a porous silicon platform, proving that the change of the refractive index of the PSi biosensor can be increased significantly if the conventional biomolecule capture detection mechanism is replaced with the high-contrast reporter cleavage mechanism [26,27]. The new mechanism makes use of the biosensor surface functionalization with high-index nanoscale reporters (e.g., quantum dots, semiconductor or noble-metal nanoparticles), which are then cleaved from the surface by a cleaving agent (e.g. a CRISPR complex) activated by the target biomolecule. [26,27]. In this work, taking advantage of the HCCD mechanism, we use a large-scale computational optimization approach called “inverse design" to engineer and optimize a highly-efficient diffraction grating that has a distinctly different optical response in the negative- and positive-result states (i.e., corresponding to the sensor effective refractive index values before cleavage and after cleavage of high-contrast reporters). The optimized diffraction grating shows 222 % (2D freeform) and 55 % (1D grating) intensity changes with a single-frequency light incident and detected at a fixed angle, ${12}^{\circ}$ (2D freeform) and ${24}^{\circ}$ (1D grating). Furthermore, the far-field intensity of the diffraction changes linearly with the number of the cleaved probes, which can be used to monitor the kinetics of the cleavage processes and to estimate the number of target molecules in the sample. This simple grating-type sensor, which can be easily re-designed for other porous materials such as e.g., anodized alumina or porous polymers, can be used to develop a simple and low-cost optical biosensor system for the point-of-care detection of known and emerging pathogens, including SARS-CoV-2 virus.
2. Inverse design
Figures 1(a)-(c) shows a schematic depiction of the HCCD biosensing approach and its integration with the inverse-designed diffraction-grating PSi biosensor. The grating structure to be optimized is composed of the nanostructured PSi layer backed by the distributed Bragg reflector (DBR) dielectric mirror to achieve efficient reflectance of incident light while minimizing the mirror dissipative losses. The DBR mirror composed of TiO$_2$ and SiO$_2$ layers has been optimized to exhibit an optical bandgap centered at the operating wavelength of 600 nm (cf. Appendix for the detailed design parameters). The Bragg mirror offers high reflection with a negligible parasitic absorption while a Si substrate offers a simple fabrication process but higher parasitic absorption and lower reflection. The other alternative is a metallic mirror, which offers high reflectivity. We believe that other types of back reflectors mentioned above may work very well with our proposed inverse design scheme, but they may have slightly lower efficiency due to lower reflectance and/or parasitic absorption. In its initial state shown in Fig. 1(b), the biosensor PSi surface is functionalized by high-contrast nanoparticle (NP) reporters (e.g., quantum dots or metal nanoparticles), attached to the sensor by the deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) tethers. The surface functionalization with the reporters can be achieved by using well-known hierarchical organic molecule layers, such as e.g., (3-Aminopropyl)triethoxysilane (APTES)-glutaraldehyde-streptavidin molecular complexes in the case of silicon and glass sensor surfaces [28,29]. After sensor exposure to the target biomolecule in the presence of cleaving agents (e.g., CRISPR-Cas12a complexes [30]), some of the reporters are collaterally cleaved by the activated agents and can be washed away (Fig. 1(c)). This results in the changes of the effective refractive index of the PSi material in the sensor final (positive-result) state. In the case of the negative result, when no target biomolecule is present in the tested sample, the reporter cleavage does not happen, and the refractive index of the sensor does not change. HCCD mechanism offers an opportunity to achieve a significant effective refractive index change by making use of the collateral cleavage of multiple reporters by an agent activated by a single target biomolecule [31,32].
Nevertheless, designing a high-efficiency two-state diffraction grating is still challenging due to the lack of a well-developed universal design methodology for multi-state diffraction gratings engineering. Furthermore, the effective refractive index change of the PSi material may still not be significant enough even when large number of reporters is cleaved. For example, freshly fabricated porous Si layer with 70 % porosity exhibits an effective refractive index of 1.637 at the wavelength of 600 nm. This estimate is based on the Bruggeman’s model using the silicon’s refractive index ($n_{Si}$) of 3.948 at 600 nm, and confirmed by experiments [26]:
The oxidation of silicon significantly lowers the PSi layer refractive index (e.g., the effective index value of the oxidized silicon layer (30% oxidized silicon/70% air) of 1.480 has been experimentally estimated [26]). The effective refractive index of the oxidized porous Si layer is further progressively reduced with the increased porosity of the material (see Eq. (1)). Finally, attachment of organic molecules to tether the reporters to the sensor surfaces further slightly increases the effective refractive index of the multi-material porous layer. For example, we have recently reported the final effective refractive index value of the PSi layer with 73% initial porosity to be as low as 1.2849 [26] after oxidization.
Since functionalized PSi has a significantly lower refractive index value than the materials typically used to create diffraction gratings (e.g., metals or high-index semiconductors), it is non-trivial to design even a single-state diffraction grating with any of the commonly-used design methods, including the geometric phase-gradient method [33,34], blazed grating method [35], and unit-cell optimization [36,37]. Other porous dielectric materials such as e.g. anodized alumina or porous polymers, will have even lower effective refractive indices [38].
To address this challenge, we apply the inverse design method to engineer a two-state diffraction grating with the low index material. Inverse design computes gradients with respect to structural degrees of freedom by “adjoint” calculation. This approach has been adapted from control theory [39–42], circuit theory [43], mechanics [44], aerodynamics [45], and deep learning [46–48] in the form of “backpropagation”. Recently, it has been introduced to nanophotonics and used to engineer and optimize waveguide demultiplexers [49–51], photonic bandgap structures [52], beam deflectors [53,54], solar cells [55], and tunable metasurfaces [56]. This method offers great advantage in designing multi-state metasurfaces, because it can simultaneously average over the figure of merits for multiple states [56]. The key metric of the optimization can be described as:
As illustrated in Figs. 1(b) and (c), the initial and the positive-test final states of our biosensor are chosen as (1) a PSi layer functionalized with gold (Au) nanoparticle-labeled reporters anchored to the Si surfaces by DNA or RNA tethers [27] and (2) a PSi layer after the Au nanoparticle reporters have been cleaved from its surface as a result of the exposure to the target biomaterial that activates the cleaving agent. The detailed measurement and estimation of the effective refractive indices of the initial and the final states can be found in the previous work [27] and Appendix. In the case of the negative test result, the initial state of the diffraction grating is not expected to change.
We use MEEP [59], open-source finite-difference time-domain method, to calculate forward and adjoint simulations of the biosensors. We employ Bloch boundary conditions at the edges of the FDTD unit cell and the transverse magnetic plane wave as the excitation optical field.
3. Diffractive biosensor optimization
We first explore an optimal design in the 2D freeform structure where we do not impose any structural constraint except the minimum feature size (30 nm). This will allow us to estimate the best theoretically achievable device performance under the HCCD sensing scenario. We assume a two-dimensional structure with a unit-cell periodicity of 4.8 $\lambda$ (2.88 $\mu m$) and a thickness of 1.5 $\lambda$ (0.9 $\mu m$) as shown in Figs. 1(b) and (c). The structure periodicity was chosen to be large enough to secure enough degrees of freedom for the optimization procedure while allowing accurate and efficient FDTD calculations of the unit cell optical response. At the same time, the first and second diffraction orders associated with this larger periodicity have diffraction angles greater than ${10}^{\circ}$, which ensures that the far-field diffraction pattern can be resolved clearly. The values of the dielectric permittivities used in this study are taken from prior experimental measurements, and include: silicon [60], Au [60], and biomaterials used to tether the Au nanoparticles to the sensor surface (APTES [61], glutaraldehyde [62] streptavidin [63]), respectively. The illuminating optical field has been chosen in the form of a transverse magnetic plane wave incident normally to the sensor surface. Although we use a 30-nm grid spacing ($\lambda$/20), the optimized structures have been validated up to ($\lambda$/120) grid-spacing to ensure numerical convergence of the algorithm (cf. Appendix).
Figure 2 shows an optimized 2D freeform PSi biosensor. The dominant order switches from 1$_{\textrm {st}}$ order to 0$_{\textrm {th}}$ order if the nanoparticle reporters are cleaved from the sensor surface. At the 0$_{\textrm {th}}$ order reflection angle, the calculated field intensity change is from 22 % to 66 %, while at the 1$_{\textrm {st}}$ order angle it changes from 58 % to 18 %. Note that this change is observed at the constant wavelength of 600 nm, thus there is no need for measuring and processing broadband reflectance spectra to capture either resonance frequency shifts or complicated phase changes. Figure 2(b) demonstrates inverse design iterations and the corresponding intermediate structures. The effective refractive indices of the PSi layer (1.0 % Au volume fraction) are estimated to be 1.2849 (after cleavage), 1.2470 (before cleavage), respectively. The structure at the fifth iteration shows an intermediate multi-layer-type geometry in grayscale, which may greatly improve total reflectivity due to DBR-like behavior, while creating different reflection fields for the two sensor states. From the 20$_{\textrm {th}}$ iteration to the final iteration, the intermediate geometry gradually becomes a binary structure with a penalization method [64]. The final structure has a disconnected multi-layer type geometry combined with 2D freeform objects. This freestanding structure is impossible to fabricate, but it provides an estimate of the theoretical optimization range, which is large enough to make possible engineering practical PSi gratings making use of the HCCD mechanism. Realistic structures with the switching response within this range can then be optimized by imposing additional constrains to the structure geometry.
To reveal the physical mechanism that drives the diffraction order switching caused by a small index change in the inverse-designed structure, we calculated the field intensity distribution (cf. Appendix, Fig. 10) and the near-field optical powerflow patterns inside the PSi layer. The optical powerflow pattern is illustrated by plotting the intensity and the phase distribution of the Poynting vector as shown in Appendix Fig. 10 and Fig. 2(d), respectively. By comparing the Poynting vector phase distributions before and after the cleavage event, it can be seen that prior to cleavage, the near-field interference field features multiple optical vortices (i.e., areas or optical powerflow circulation forming around the phase singularities - points of total destructive interference of the electromagnetic field) [65–68]. The small change in the effective refractive index caused by the probes cleavage is however large enough to change the interference pattern and annihilate most of the vortex pairs, while re-positioning others. This causes drastic changes in the near-field optical powerflow pathways, and, in turn, results in the switching of the diffraction order in the far field of the sensor. These large variations of the optical response of the structure caused by a relatively small perturbation stem from the topological nature of optical vortices, which appear or annihilate in pairs, causing major re-distribution of the powerflow as a result of the vortex emergence or annihilation events.
We used a realistic fabrication constraint by reducing the acceptable sensor designs to the two dimensional ridge-type grating structures composed of ridges of constant height and minimum width of 30 nm, distributed on the surface without any wavelength-scale periodicity required. The optimal device, shown in Fig. 3, has the thickness of 0.54 $\mu m$ and the same large-scale (i.e., FDTD unit cell) periodicity as the 2D freeform structure. Unlike the case of a 2D freeform design, the greater designable volume is not necessarily beneficial for optimizing a realistic grating structure for the highest efficiency. Therefore, we chose the thickness of the PSi grating such that it will be amenable to fabrication by standard lithographic techniques. The effective refractive indices of the PSi layer (2.0 % Au volume fraction) are estimated to be 1.2849 (after cleavage), 1.2133 (before cleavage), respectively. The optimal device shown in Fig. 3(a) has the grating thickness of 540 nm and exhibits switching of the reflected light from 2$_{\textrm {nd}}$ order to 0$_{\textrm {th}}$ order upon cleavage of the nanoparticle-labeled reporters. At the 0$_{\textrm {th}}$ order reflection angle, the intensity change is from 24 % to 47 % while at the 2$_{\textrm {nd}}$ order angle it changes from 42 % to 27 %.
To relate this diffraction efficiency to the real experiment using a single-frequency laser light as the excitation field instead of a planewave, Figs. 3(d) and (e) demonstrate the far-field optical field intensity profile under the Gaussian beam incidence. The intensity switching between the 2$_{\textrm {nd}}$ order and 0$_{\textrm {th}}$ can be clearly seen without any post-processing method. The optimal structure shown in Fig. 3(c) is composed of two repeating similar structures (they are off by one parameter) within the given periodicity (2.88 $\mu m$). This effect makes the diffraction powers being concentrated on the even diffraction orders (-4, -2, 0, 2, 4), whose angles match with a -2,-1,0,+1,2 orders of the half-periodicity (1.44 $\mu m$). Figure 3(b) demonstrates the inverse design iterations and the corresponding intermediate structures.
Similar to the case of the freeform structure design, we investigated the changes in the near-field optical powerflow pattern than drive the observed changes in the far-field diffraction pattern. The Poynting vector phase profile shown in Fig. 3(f) features a few optical phase singularities with the associated optical vortices driving the powerflow in the near field of the grating. Upon cleavage, some of this singularities shift and annihilate each other, resulting in the switching of the grating far-field diffraction pattern. Note that a similar vortex-driven optical switching mechanism is behind phase transitions of hyperbolic metamaterials [68], plasmonic nanolensing effect [69,70], and the enhanced light transmission through plasmonic nanohole arrays [65].
Although both the 2D freeform and the 1D grating optimized configurations demonstrate promising sensing performances, attaching the exact number of probes could be very challenging experimentally [26]. Thus, we evaluate the device sensitivity to the variations in the surface functionalization coverage by calculating the far-field field intensity profiles for different volume fractions of the Au probes in the PSi material, as shown in Fig. 4. The optimized grating was designed with the assumption of 2.0 % Au volume fraction before cleavage. Our calculations show that for different Au volume fractions ranging from 1.5 % to 2.5 %, the far-field intensity varies linearly, which makes it possible to predict the initial volume fraction of Au probes by analyzing the intensity profiles. This observation proves that the optimized diffraction grating design proposed here can tolerate fabrication imperfections and varied reporter surface coverage densities, and may potentially lead to a development of biosensor capable of monitoring the probe cleavage events in real time.
4. Summary and outlook
In this work, we have demonstrated an inverse design approach to develop a new diffractive-type single-frequency fixed-incidence angle porous silicon biosensing platform with the vertical optical readout mechanism suitable for real-time point-of-care applications. We focused on grating-like designs in 2D with two schemes (2D freeform and 1D grating). The former configuration provided an estimate for the upper limit of the sensing performance, while the latter yielded a lithography-compatible practical sensor geometry with a linear intensity change expected as a result of the probe cleavage. We attribute the switchable behavior of the inverse-designed sensors to the proper engineering of the near-field optical powerflow patterns driven by optical vortices, which are re-configured as a result of the probe cleavage event. A natural extension of this work would be to implement an inverse design technique in combination with the high contrast reporter cleavage detection approach to inverse-design an on-chip integrated interferometric sensor platform to further simplify optical readout mechanism in a compact portable sensor configuration. The new types of inverse-designed sensor platforms making use of the HCCD mechanism hold high promise for the development of simple yet accurate point-of-care biosensors, which are easily programmable for detection of different pathogens, including SARS-Cov-2 virus.
5. Appendix
In the Appendix, we will discuss the convergence study (Figs. 5, 6, and 7), the robustness study (Figs. 8 and 9), and the phase analysis of the Poynting vector (Figs. 10 and 11).
5.1. Convergence study
5.2. Robustness study
5.3. Phase of the Poynting vector
The x,y components of the Poynting vector field can be calculated with:
5.4. Geometrical parameters
Funding
U.S. Army Combat Capabilities Development Command Soldier Center (W911NF-13-D-0001).
Acknowledgements
The authors thank Michael Dubrovsky and Diedrik Vermeulen (SiPhox), Ke Du (Rochester Institute of Technology), Rabeb Layouni and Sharon M. Weiss (Vanderbilt University) for useful discussions. The authors also thank Owen D. Miller (Yale University) for providing insight and computational resources.
Disclosures
The authors declare no conflicts of interest.
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