Abstract
We demonstrate that picosecond time-gated fluorescence microscopy can be used to monitor subtle changes in the kinetics and spatial distribution of perturbations to the molecular and cellular structure of plant tissue caused by ultraviolet radiation. Single-molecule experiments on Photosystem II and chloroplast preparations give picosecond fluorescence decay kinetics that are similar to those obtained previously on bulk samples. For green plant leaves, localized and well-defined cellular structure is seen for normal material whereas relatively diffuse and non-specific features are seen after UV-irradiation indicating significant UV-induced rupture of the cellular structure. The changes in the chlorophyll fluorescence decay kinetics indicate uncoupling of chlorophyll molecules in the light-harvesting system leading to inhibition of energy reorganization and transfer in the antennae and subsequent exciton transfer to the reaction centers.
©2002 Optical Society of America
Full Article | PDF ArticleMore Like This
Xiaotong Yuan, Andrew Bowling, Erin Gemperline, Varun Mannam, and Scott Howard
Opt. Express 32(8) 13733-13745 (2024)
Ana Celia Muñoz-Muñoz, Humberto Gutiérrez-Pulido, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay, and Jesús Cervantes-Martínez
Appl. Opt. 46(11) 2138-2142 (2007)
Jesús Cervantes-Martínez, Ricardo Flores-Hernández, Benjamín Rodríguez-Garay, and Fernando Santacruz-Ruvalcaba
Appl. Opt. 41(13) 2541-2545 (2002)